How Embryologists Use a Vit Kit for Safe and Reliable Cryopreservation

LONDON - EntSun -- Vitrification is one of the most critical steps in assisted reproductive technology (ART). For embryologists, the goal is to ensure that gametes and embryos remain viable and structurally intact through freezing and thawing cycles. The vit kit plays a central role in achieving that reliability and precision.

A vit kit generally includes a high-security straw, a capillary tube with a pre-formed gutter, coloured handling rods, and a plastic insertion device. Each component is designed for sterile, contamination-free manipulation and storage of reproductive cells.

The process begins with sample preparation, where oocytes or embryos are equilibrated in vitrification solutions according to laboratory protocols. Using the coloured handling rod, the embryologist then gently transfers each sample into the ionomeric resin straw through the pre-formed gutter. This design not only facilitates precise placement but also prevents loss or contamination during loading.

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Once the sample is positioned, vitrification takes place a rapid cooling step that transforms the medium into a glass-like solid without ice crystal formation. This step is crucial, as even minimal crystallisation can damage cell membranes and compromise viability. The vitrified sample is then sealed using a compatible manual or automatic sealer, ensuring complete security of the straw.

Following sealing, samples are stored in liquid nitrogen tanks or vapour-phase dry shippers for long-term preservation and transport. The integrity of the straw and the sterility of all materials used are essential to maintaining the biological safety of the specimen throughout its lifecycle.

In practice, experienced embryologists understand that vitrification success depends not only on the equipment but also on the precision of technique. Every step from loading to sealing must be executed with care, consistency, and adherence to validated lab protocols. (https://cryolab.co.uk/product/cbs-high-security-vitrification-kit/)

Cryolab supplies the CBS High Security Vit Kit, trusted in IVF and research laboratories worldwide for its reliability, sterility, and compatibility with CBS sealing systems. When paired with a CBS SYMS Sealer, the vit kit provides a complete solution for secure, traceable cryopreservation.