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EntSun News/11107840
The guide follows published ATCC and ECACC guidance from culture checks to thawing, and sets out where each step can go wrong, including the transfer from freezer to vapour phase storage.
WEST SUSSEX, U.K. - EntSun -- Cryolab, a specialist supplier of cryogenic equipment, consumables and servicing to laboratories worldwide, has published a practical guide to cell cryopreservation for cell culture, cell banking and biobanking teams.
The guide sets out a standard cell freezing protocol for mammalian cell lines, based on published guidance from the ATCC and ECACC culture collections, and then looks at the equipment behind each step. It is written for laboratory managers and scientists who already freeze cells and want more consistent recovery.
Topics covered include:
- Checks before freezing: log phase growth, viability above 90%, contamination and identity testing
- Freeze medium and cell concentration, with DMSO at 5% to 10% as the usual cryoprotectant
- Cooling at a controlled rate, and how a controlled-rate freezer compares with a passive freezing container
More on EntSun News
- The transfer from the freezing step to storage, which the ATCC identifies as a major cause of variation in viability on thawing
- Storage below -130 °C in the vapour phase of a liquid nitrogen vessel
- Vial labelling and location records
- Rapid thawing and removal of the cryoprotectant
- Splitting valuable cultures between at least two storage locations
The guide also explains how storage scales with vial numbers, from cryocanes in a small storage vessel to large steel storage tanks holding from 15,600 to 95,000 vials in racks of boxes.
The guide is free to read on the Cryolab website. Laboratories should always follow their own validated methods and the data sheet for each cell line.
Read the guide: https://cryolab.co.uk/cell-cryopreservation-freezing-protocol/
About Cryolab
Cryolab was established in 2000 and is based in Chichester, England. The company supplies cryogenic equipment, consumables, accessories and servicing to IVF clinics, hospitals, biobanks and research laboratories worldwide, and holds ISO 9001:2015 certification.
The guide sets out a standard cell freezing protocol for mammalian cell lines, based on published guidance from the ATCC and ECACC culture collections, and then looks at the equipment behind each step. It is written for laboratory managers and scientists who already freeze cells and want more consistent recovery.
Topics covered include:
- Checks before freezing: log phase growth, viability above 90%, contamination and identity testing
- Freeze medium and cell concentration, with DMSO at 5% to 10% as the usual cryoprotectant
- Cooling at a controlled rate, and how a controlled-rate freezer compares with a passive freezing container
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- The transfer from the freezing step to storage, which the ATCC identifies as a major cause of variation in viability on thawing
- Storage below -130 °C in the vapour phase of a liquid nitrogen vessel
- Vial labelling and location records
- Rapid thawing and removal of the cryoprotectant
- Splitting valuable cultures between at least two storage locations
The guide also explains how storage scales with vial numbers, from cryocanes in a small storage vessel to large steel storage tanks holding from 15,600 to 95,000 vials in racks of boxes.
The guide is free to read on the Cryolab website. Laboratories should always follow their own validated methods and the data sheet for each cell line.
Read the guide: https://cryolab.co.uk/cell-cryopreservation-freezing-protocol/
About Cryolab
Cryolab was established in 2000 and is based in Chichester, England. The company supplies cryogenic equipment, consumables, accessories and servicing to IVF clinics, hospitals, biobanks and research laboratories worldwide, and holds ISO 9001:2015 certification.
Source: Cryolab Ltd
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